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Regional Reference Centre for Virological Surveillance of Influenza  

Principal Investigator:

Prof.ssa Elena Pariani


Mr. Anselmi Giovanni
Dr.ssa Cristina Galli
Dr.ssa Laura Pellegrinelli

The group’s research activity is focused on:

The Reference Centre for virological surveillance of influenza in Lombardy operates within the national surveillance network InfluNet. The center, which is equipped with multi-purpose laboratories, has been validated and accredited by the National Influenza Centre (NIC) for the diagnosis of epidemic/pandemic viruses and avian viruses with pandemic potential.

The main purpose of the surveillance is the characterization of different types and subtypes of influenza viruses circulating in the geographical area of interest. The research group carries out molecular and phylogenetic studies designed to monitor viral evolution, to identify the emergence of drug-resistant viral variants and to assess the homology between vaccine strains and circulating viruses. The results of these investigations are promptly sent to the NIC, which makes them available to both national reference agencies (Ministry of Health) and international ones (World Health Organization), providing an essential contribution to the annual update of vaccines.


Currently, the research has its primary focus on two major research lines:

- Topic 1. Molecular epidemiology and prevention of respiratory viruses and, in particular, influenza viruses, including:

    - virological and molecular characterization of circulating respiratory viruses;

    - development of diagnostic algorithms for the early detection of emerging viruses with pandemic potential;

    - phylogenetic analysis aimed at the study of evolutionary mechanisms and epidemiological dynamics of respiratory viruses.

- Topic 2. Evaluation of immunogenicity of influenza vaccination in groups at risk of complications, such as people with chronic diseases, pregnant women and immunocompromised patients.


Recent/relevant Publications

  1. AmendolaA, ParianiE, ViganòA, ErbaP, Zappa A, GiacometV, TanziE, ZuccottiGV, Zanetti A. Influenza surveillance in a cohort of HIV-infected children and adolescents immunized against seasonal influenza. Vaccine, 2010;28(15):2700-4. ISSN:0264-410X. PMID:20105427.
  2. Orlando G, Pariani E, Mazza F, Tanzi E, Meraviglia P, Gianelli E, Argenteri B, Amendola A, Galli M, Rizzardini G, Zanetti A. Pandemic influenza vaccine in adult HIV-1-infected patients. AIDS, 2010;24(13):2142-3. ISSN:1525-4135. PMID:20679764.
  3. ZuccottiGV, PoglianiL, ParianiE, Amendola A, Zanetti A. Transplacental antibody transfer following maternal immunization with a 2009 A/H1N1v pandemic influenza MF59-adjuvanted vaccine. JAMA, 2010;304(21):2360-1. ISSN:0098-7484. PMID:21119083.
  4. Pariani E, Boschini A, AmendolaA, PolettiR, AnselmiG, BegniniM, RanghieroA, CecconiG, Zanetti A. Response to 2009 pandemic and seasonal influenza vaccines co-administered to HIV-infected and HIV-uninfected former drug users living in a rehabilitation community in Italy. Vaccine, 2011;29(49):9209-13. ISSN:0264-410X. PMID:21974995. (IF=3,572)
  5. Zehender G, Pariani E, Piralla A, Lai A, Gabanelli E, Ranghiero A, Ebranati E, Amendola A, Campanini G, Rovida F, CiccozziM, Galli M, Baldanti F, Zanetti AR. Reconstruction of the evolutionary dynamics of the A(H1N1)pdm09 influenza virus in italy during the pandemic and post-pandemic phases. PLoS One, 2012. 7(11):e47517. ISSN:1932-6203. PMID:22194826.



-   Prof. Alessandro Zanetti

Phone: 02 503 15126, email: alessandro.zanetti@unimi.it

-  Dr. Antonella Amendola

Phone: 02 503 25072, email: antonella.amendola@unimi.it

-  Mr. Giovanni Anselmi

Phone: 02 503 15127, email: giovanni.anselmi@unimi.it

-  Dr. Elena Pariani

Phone: 02 503 15122, email: elena.pariani@unimi.it

-  Dr. Alberto Ranghiero

Phone: 02 503 15122, email: alberto.ranghiero@unimi.it


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